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. 1977 Mar 25;195(4284):1328-30.
doi: 10.1126/science.841330.

Kinetic resonance Raman spectroscopy: dynamics of deprotonation of the Schiff base of bacteriorhodopsin

M A Marcus, A Lewis

Kinetic resonance Raman spectroscopy: dynamics of deprotonation of the Schiff base of bacteriorhodopsin

M A Marcus et al. Science. .

Abstract

We have developed a kinetic technique, combining resonance Raman spectroscopy and variable-speed continuous flow methods, to study molecular dynamics of isolated sites in macromolecules. Kinetic resonance Raman spectra of the retinylidence chromophore of bacteriorhodopsin have been obtained and the dynamics of the deprotonation of the Schiff base linkage is discussed.

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