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. 1993 Oct 14;365(6447):661-3.
doi: 10.1038/365661a0.

The GTP-binding protein Ran/TC4 is required for protein import into the nucleus

M S Moore  1 , G Blobel
Affiliations

The GTP-binding protein Ran/TC4 is required for protein import into the nucleus

M S Moore et al. Nature. .

Abstract

Two cytosolic fractions (A and B) from Xenopus oocytes are sufficient to support protein import into the nuclei of digitonin-permeabilized cells. Fraction A recognizes the nuclear localization sequence (NLS) and binds the import substrate to the nuclear envelope, whereas fraction B mediates the subsequent passage of the bound substrate into the nucleus. Here we report that two interacting components are required for full fraction-B activity, purify one of these components to homogeneity, and show that it is the highly abundant GTP-binding protein Ran (Ras-related nuclear protein)/TC4.

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