Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1993 Oct 14;365(6447):671-3.
doi: 10.1038/365671a0.

Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing

B A Larder  1 , A Kohli, P Kellam, S D Kemp, M Kronick, R D Henfrey
Affiliations
Comparative Study

Quantitative detection of HIV-1 drug resistance mutations by automated DNA sequencing

B A Larder et al. Nature. .

Abstract

A comparison has been made between manual and automated DNA sequencing procedures to evaluate the ability to distinguish mixtures of wild-type and mutant sequences. Quantitative detection of such mixtures of HIV-1 drug resistance mutations was best achieved using an automated system that uses fluorescent-labelled sequencing primers. This procedure has a wide range of applications in clinical research, including heterozygote analysis. Software that automatically reports mixed-base positions is presented.

PubMed Disclaimer

Publication types

LinkOut - more resources