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Comparative Study
. 1993 Jan;31(1):152-4.
doi: 10.1128/jcm.31.1.152-154.1993.

Detection of Francisella tularensis in blood by polymerase chain reaction

G W Long  1 , J J Oprandy, R B Narayanan, A H Fortier, K R Porter, C A Nacy
Affiliations
Comparative Study

Detection of Francisella tularensis in blood by polymerase chain reaction

G W Long et al. J Clin Microbiol. 1993 Jan.

Abstract

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

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