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Comparative Study
. 1993 Jan 15;190(1):289-93.
doi: 10.1006/bbrc.1993.1044.

Molecular cloning of the smaller subunit(P52) of rat liver mitochondrial processing protease

S Kitada  1 , T Niidome, T Nagano, T Ogishima, A Ito
Affiliations
Comparative Study

Molecular cloning of the smaller subunit(P52) of rat liver mitochondrial processing protease

S Kitada et al. Biochem Biophys Res Commun. .

Abstract

A cDNA encoding the smaller subunit (P52) of mitochondrial processing protease was isolated from a rat liver cDNA library, using cDNA fragment for yeast MAS1 as the probe. The deduced amino acid sequence is highly homologous to those of PEP from Neurospora crassa and MAS1 from Saccharomyces cerevisiae. After in vitro transcription and translation, the precursor peptide was imported into isolated rat liver mitochondria and processed to its mature form.

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