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Comparative Study
. 1993 Jan 15;289 ( Pt 2)(Pt 2):605-7.
doi: 10.1042/bj2890605.

Sequence-specific binding of [N-MeCys3,N-MeCys7]TANDEM to TpA

M Lavesa  1 , R K Olsen, K R Fox
Affiliations
Comparative Study

Sequence-specific binding of [N-MeCys3,N-MeCys7]TANDEM to TpA

M Lavesa et al. Biochem J. .

Abstract

The sequence selective binding of [N-MeCys3,N-MeCys7]TANDEM to DNA has been studied by footprinting experiments on DNA fragments containing the self-complementary sequences CGCGATATCGCG, CGCGTATACGCG, CGCGTTAACGCG and CGCGAATTCGCG. DNAase I and micrococcal nuclease reveal drug-induced footprints with the central sequences ATAT, TATA and TTAA, but not AATT, suggesting that the ligand binds to the dinucleotide TpA. The ligand renders certain adenines hyper-reactive to diethyl pyrocarbonate. These are observed with ATAT, TATA and TTAA, but not AATT, and are located both within, and distal to, the TpA-binding sites.

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