Cloning, sequencing and expression of the mouse mammalian achaete-scute homolog 1 (MASH1)

Abstract

We describe the cloning of a full length cDNA encoding the mouse mammalian achaete-scute homolog 1 (mouse MASH1). Using a ribonuclease protection assay to examine expression of this gene in cell lines, postimplantation embryos and adult tissues, expression was detected between days 10.5 and 16.5 of gestation and in adult brain. No expression was detected in other adult tissues or in most of the cell lines examined. However, differentiation of P19 embryonal carcinoma cells into neuronal cell types by exposure to retinoic acid resulted in the induction of MASH1 RNA expression.