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. 1993 Jan;21(1):181-3.
doi: 10.1007/BF00039629.

Rapid identification of Triticeae genotypes from single seeds using the polymerase chain reaction

C Benito  1 , A M Figueiras, C Zaragoza, F J Gallego, A de la Peña
Affiliations

Rapid identification of Triticeae genotypes from single seeds using the polymerase chain reaction

C Benito et al. Plant Mol Biol. 1993 Jan.

Abstract

An easy and quick protocol has been developed for DNA analysis via PCR. Single cereal endosperm or small leaf pieces can be separately processed in several PCR reactions. The resultant PCR patterns are equivalent to those obtained with standard DNA extraction protocols using either specific or random primers. Intra- and inter-specific variability can be detected. This method allows the analysis of a large number of individuals in early stages prior to the plant sowing.

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References

    1. Plant Mol Biol. 1990 Jul;15(1):169-71 - PubMed
    1. Nucleic Acids Res. 1990 Nov 25;18(22):6531-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2336-40 - PubMed
    1. Theor Appl Genet. 1991 Oct;82(5):627-32 - PubMed
    1. Nucleic Acids Res. 1990 Dec 25;18(24):7213-8 - PubMed

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