Observations by scanning electron microscopy of oviductal epithelial cells from cows at follicular and luteal phases

Abstract

The luminal surfaces of epithelial cells in various regions of the bovine oviduct from cows, at the follicular and luteal phases of the estrous cycle, were examined by scanning electron microscopy. Marked cyclic changes were observed on the surface of the epithelium in the fimbriae and ampulla, but few changes were found in the isthmus and uterotubal junction. The epithelium of the fimbriae and ampulla of oviducts in the follicular phase were densely ciliated, and the cilia concealed the apical processes of the nonciliated cells. In the luteal phase, the nonciliated cells predominated in the epithelium and most of the ciliated cells were hidden by the bulbous processes of the nonciliated cells. The epithelium of the ampullar-isthmic junction showed similar changes, but to a lesser extent. In the isthmus and at the utero-tubal junction, the apical surfaces of the nonciliated cells were flat or gently rounded during the estrous cycle. Quantitative examinations by light microscopy showed that the mean percentage of ciliated cells significantly decreased in the fimbriae and ampulla at the luteal phase, but not in the other regions. The height of ciliated cells decreased dramatically in the fimbriae, ampulla, and ampullar-isthmic junction at the luteal phase. By contrast, the height of nonciliated cells decreased significantly in the ampullar-isthmic junction, isthmus, and utero-tubal junction at the luteal phase, but not in the fimbriae and ampullae. The results demonstrate that there are regional variations and cellular differences in the cyclic changes associated with the oviductal epithelial cells in the cow.