doi: 10.1007/BF00446662.
Selective spore survival during replica-plating of fission yeast
- PMID: 843164
- DOI: 10.1007/BF00446662
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Selective spore survival during replica-plating of fission yeast
Arch Microbiol.
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Abstract
Vegetative cells of Schizosaccharomyces pombe, upon exposure to acetone vapours, are inactivated at a faster rate than ascospores of this yeast. This observation has been used to develop a simple and fast method by which colonies can be replica-plated and only spores survive in the replicas. The colony patterns are exposed to acetone while still on the velvet used for replica-plating.
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