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. 1977 Feb 4;112(1):109-10.
doi: 10.1007/BF00446662.

Selective spore survival during replica-plating of fission yeast

R Egel

Selective spore survival during replica-plating of fission yeast

R Egel. Arch Microbiol. .

Abstract

Vegetative cells of Schizosaccharomyces pombe, upon exposure to acetone vapours, are inactivated at a faster rate than ascospores of this yeast. This observation has been used to develop a simple and fast method by which colonies can be replica-plated and only spores survive in the replicas. The colony patterns are exposed to acetone while still on the velvet used for replica-plating.

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References

    1. Schweiz Z Pathol Bakteriol. 1957;20(5):535-44 - PubMed
    1. Mol Gen Genet. 1974;131(4):281-9 - PubMed

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