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. 1993 Mar;60(3):1175-8.
doi: 10.1111/j.1471-4159.1993.tb03273.x.

Stable expression and regulation of a rat brain K+ channel

S D Critz  1 , B A Wible, H S Lopez, A M Brown
Affiliations

Stable expression and regulation of a rat brain K+ channel

S D Critz et al. J Neurochem. 1993 Mar.

Abstract

The Shaw-type K+ channel Kv3.1 was stably transfected in human embryonic kidney cells. Voltage dependence of activation, K+ permeability, sensitivity to external tetraethylammonium, and unitary conductance were similar to Kv3.1 channels expressed transiently in Xenopus oocytes. Kv3.1 channels appear to be regulated because the protein kinase C activator phorbol 12,13-dibutyrate decreased Kv3.1 currents. Based on these results, we find that the stable expression of voltage-gated K+ channels in human embryonic kidney cells appears to be well suited for analysis of both biophysical and biochemical regulatory processes.

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