Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134
- PMID: 8439238
- DOI: 10.1007/BF00250280
Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134
Abstract
2,4-Dichlorophenoxyacetate (2,4-D) in Alcaligenes eutrophus JMP134 (pJP4) is degraded via 2-chloromaleylacetate as an intermediate. The latter compound was found to be reduced by NADH in a maleylacetate reductase catalyzed reaction. Maleylacetate and chloride were formed as products of 2-chloromaleylacetate reduction, the former being funneled into the 3-oxoadipate pathway by a second reductive step. There was no indication for an involvement of a pJP4-encoded enzyme in either the reduction or the dechlorination reaction.
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