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. 1993;159(2):182-8.
doi: 10.1007/BF00250280.

Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134

M D Vollmer  1 , K Stadler-Fritzsche, M Schlömann
Affiliations

Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134

M D Vollmer et al. Arch Microbiol. 1993.

Abstract

2,4-Dichlorophenoxyacetate (2,4-D) in Alcaligenes eutrophus JMP134 (pJP4) is degraded via 2-chloromaleylacetate as an intermediate. The latter compound was found to be reduced by NADH in a maleylacetate reductase catalyzed reaction. Maleylacetate and chloride were formed as products of 2-chloromaleylacetate reduction, the former being funneled into the 3-oxoadipate pathway by a second reductive step. There was no indication for an involvement of a pJP4-encoded enzyme in either the reduction or the dechlorination reaction.

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