The pRSET family of T7 promoter expression vectors for Escherichia coli

Abstract

A family of eight T7 promoter-based expression plasmids is presented. These are high-copy-number vectors featuring translational start and stop elements and a multiple cloning site (polylinker) with eleven unique restriction sites in all six reading frames. Depending on the cloning strategy used, recombinant proteins may contain either short vector-encoded fusion fragments or no fusion fragments at all. Following promoter induction, proteins are usually produced at a high level.