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. 1993 Jan 11;21(1):5-12.
doi: 10.1093/nar/21.1.5.

Different TBP-associated factors are required for mediating the stimulation of transcription in vitro by the acidic transactivator GAL-VP16 and the two nonacidic activation functions of the estrogen receptor

C Brou  1 , J Wu, S Ali, E Scheer, C Lang, I Davidson, P Chambon, L Tora
Affiliations
Free PMC article

Different TBP-associated factors are required for mediating the stimulation of transcription in vitro by the acidic transactivator GAL-VP16 and the two nonacidic activation functions of the estrogen receptor

C Brou et al. Nucleic Acids Res. .
Free PMC article

Abstract

The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonstrate that a factor(s) required for the function of the ER AFs is chromatographically separable from a factor(s) necessary for the activity of the acidic activation domain of VP16. Moreover, immunoprecipitation experiments using a monoclonal antibody directed against the TATA box binding protein (TBP) indicate, that these different factors are associated with TBP in distinct TFIID complexes.

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References

    1. J Biol Chem. 1982 Dec 10;257(23):14419-27 - PubMed
    1. Science. 1988 Sep 9;241(4871):1335-8 - PubMed
    1. EMBO J. 1986 Mar;5(3):553-60 - PubMed
    1. EMBO J. 1986 May;5(5):891-7 - PubMed
    1. Mol Cell Biol. 1990 Dec;10(12):6335-47 - PubMed

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