Immunohistochemistry of fibronectin and actin in ungrafted wounds and wounds covered with full- and split-thickness skin grafts

Abstract

Fibronectin and actin content were measured with immunochemistry in excised wounds in 18 rats. In six rats, wounds were allowed to granulate and contract. Wounds were grafted with full-thickness skin in six rats and with split-thickness skin grafts in the remaining six. Multiple biopsies were taken for immunochemistry and electron microscopy. Actin and fibronectin content was greatest in ungrafted wounds. This content was reduced in wounds by skin grafting, with full-thickness grafts having a generally more inhibitory effect than split. In grafted wounds, actin was observed primarily in the wound bed, whereas fibronectin was present in both the wound bed and graft. Fibronectin appeared coincidently with actin in split-thickness skin grafts and their wounds. In contrast, full-thickness skin grafts had fibronectin staining before actin. The degree of wound contraction inhibition by different types of skin grafts appears to correlate with the fibronectin and actin staining patterns.