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Comparative Study
. 1993 Mar 5;1162(1-2):227-9.
doi: 10.1016/0167-4838(93)90152-h.

Dimethyl sulfoxide favours the covalent phosphorylation and not the binding of Pi to sarcoplasmic reticulum ATPase

E Mintz  1 , V Forge, F Guillain
Affiliations
Comparative Study

Dimethyl sulfoxide favours the covalent phosphorylation and not the binding of Pi to sarcoplasmic reticulum ATPase

E Mintz et al. Biochim Biophys Acta. .

Abstract

Competition between Ca2+ binding and Pi phosphorylation showed that the affinity of Ca(2+)-ATPase for Pi was not changed by 20% DMSO. Thus, the enhancement of Pi phosphorylation in the presence of DMSO should not be attributed to a solvent effect on the affinity for Pi, but rather on the phosphorylation reaction itself.

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