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. 1977 Feb;32(2):221-6.

Endothelial adenine uptake as an assay for cell- or complement-mediated cytotoxicity

D P DeBono, D E Macintyre, D J White, J L Gordon

Endothelial adenine uptake as an assay for cell- or complement-mediated cytotoxicity

D P DeBono et al. Immunology. 1977 Feb.

Abstract

[3H] Adenine uptake by monolayers of cultured pig aortic endothelium was characterized and used as the basis for a terminal adenine uptake cytotoxicity assay. This assay was used to demonstrate specific cytotoxicity against donor-type endothelium by serum and by peripheral blood lymphocytes from skin-graft allergized pigs, and to compare the time course of these two types of effector mechanism.

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