Enzymatic assay of fructose-1,6-diphosphate for the measurement of its utilization by tissues
- PMID: 844954
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Enzymatic assay of fructose-1,6-diphosphate for the measurement of its utilization by tissues
Int J Vitam Nutr Res.
1977.
Abstract
The enzymatic analysis of fructose-1,6-diphosphate by a coupled assay utilizing triose-phosphate isomerase, glycerol-3-phosphate dehydrogenase and aldolase was carried out in rat serum and human plasma after fructose-1,6-diphosphate administration. The rate of disappearance of fructose-1,6-diphosphate was determined and compared to the activity of aspecific phosphatase. The uptake of fructose-1,6-diphosphate by rat liver and ileum was also determined with the same method.
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