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. 1993 Feb 1;208(2):237-40.
doi: 10.1006/abio.1993.1038.

Analysis of the neurotoxin beta-ODAP and its alpha-isomer by precolumn derivatization with phenylisothiocyanate

J K Khan  1 , N Kebede, Y H Kuo, F Lambein, A De Bruyn
Affiliations

Analysis of the neurotoxin beta-ODAP and its alpha-isomer by precolumn derivatization with phenylisothiocyanate

J K Khan et al. Anal Biochem. .

Abstract

A method is presented for determining 3-N-oxalyl-2,3-diaminopropanoic acid (beta-ODAP), the most potent neurotoxic substance of the seeds and seedlings of Lathyrus sativus, and its much less toxic 2-isomer (alpha-ODAP). The separation of the two forms is achieved after derivatization with phenylisothiocyanate employing a HPLC system. This method was used to monitor the isomerization of beta-ODAP to alpha-ODAP at different time intervals and to quantify the toxin level in seed extracts.

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