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. 1993 Mar 15;319(1-2):90-4.
doi: 10.1016/0014-5793(93)80043-t.

Effect of site-directed mutagenesis on conserved positions of Drosophila alcohol dehydrogenase

N Cols  1 , G Marfany, S Atrian, R Gonzàlez-Duarte
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Free article

Effect of site-directed mutagenesis on conserved positions of Drosophila alcohol dehydrogenase

N Cols et al. FEBS Lett. .
Free article

Abstract

Tyr152 and Lys156 may be functionally important residues in Drosophila ADH as they are conserved in the genus and in all short-chain dehydrogenases. In addition, unaltered Gly positions could have a crucial role in the building of the structural framework. We have modified Drosophila ADH and expressed the mutant forms in E. coli. Mutation of Tyr152 to Glu or Gln, Lys156 to Ile, Gly184 to Leu, and the double mutant Gly130 to Cys and Gly133 to Ile, all rendered, with different substrates and at different pHs, an inactive enzyme. Results suggest that Tyr152 and Lys156 are involved in catalysis and that Gly130, Gly133 and Gly184 contribute substantially to the structure of the active form.

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