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. 1993 Jan 15;106(2):139-42.
doi: 10.1111/j.1574-6968.1993.tb05949.x.

Covalently immobilized DNA plate for luminometric DNA-DNA hybridization to identify viridans streptococci in under 2 hours

S Adnan  1 , N Li, H Miura, Y Hashimoto, H Yamamoto, T Ezaki
Affiliations

Covalently immobilized DNA plate for luminometric DNA-DNA hybridization to identify viridans streptococci in under 2 hours

S Adnan et al. FEMS Microbiol Lett. .

Abstract

Single-stranded chromosomal DNA was covalently bound to a microdilution plate and used for quantitative DNA-DNA hybridization. After 30 min, hybridized DNA was quantitatively detected by alkaline phosphatase and a chemiluminescent substrate. This method was successfully used for the rapid identification of viridans streptococci.

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