Isolation and characterization of a yeast gene that is homologous with a meiosis-specific cDNA from a plant

Abstract

By using as probe a meiosis-specific cDNA clone LIM15 from the monocotyledonous plant, Lilium longiflorum, a clone containing a 2.8 kb DNA fragment was isolated from a genomic library of Saccharomyces cerevisiae. Primary structure analysis revealed that the clone includes two complete open reading frames, designated ISC2 and ISC10, capable of coding for a 36.6 kDa and a 31.6 kDa polypeptide, respectively, with the former frame being interrupted by a 92 bp intron. The predicted amino acid sequence of Isc2 was 56% identical with the putative gene product of lily cDNA clone LIM15, and showed limited sequence similarity with the yeast RAD57 gene product. Transcripts of the two genes begin accumulating 2.5 h and 7.5 h after induction of meiosis, respectively, according to a Northern hybridization analysis. Since disruption of either one of these genes had a drastic effect on the ability to form spores, ISC2 and ISC10 are expected to play significant roles in the formation of reproductive cells.