Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Mar 15;290 ( Pt 3)(Pt 3):731-4.
doi: 10.1042/bj2900731.

Effects of different enzymic treatments on the release of titin fragments from rabbit skeletal myofibrils. Purification of an 800 kDa titin polypeptide

C Astier  1 , J P Labbé, C Roustan, Y Benyamin
Affiliations

Effects of different enzymic treatments on the release of titin fragments from rabbit skeletal myofibrils. Purification of an 800 kDa titin polypeptide

C Astier et al. Biochem J. .

Abstract

In myofibrils, titin (also called connectin) molecules span from Z line to M line and constitute a third filament system containing an elastic domain in the I band. This giant protein is particularly sensitive to proteolysis in situ. Treatment of rabbit skeletal myofibrils with exogenous proteinases induces a release of titin fragments, which are detected in the soluble myofibrillar fraction. The cleavage of titin occurs at specific points localized at the proximity of Z line and could lead to a concomitant release of alpha-actinin.

PubMed Disclaimer

References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Biochem. 1992 Jun;111(6):778-82 - PubMed
    1. J Biochem. 1979 Jun;85(6):1539-42 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3698-702 - PubMed
    1. J Biochem. 1981 Mar;89(3):701-9 - PubMed

Publication types