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. 1993 Mar 15;290 ( Pt 3)(Pt 3):777-82.
doi: 10.1042/bj2900777.

The 17 beta-oestradiol dehydrogenase of pig endometrial cells is localized in specialized vesicles

J Adamski  1 , B Husen, F Marks, P W Jungblut
Affiliations

The 17 beta-oestradiol dehydrogenase of pig endometrial cells is localized in specialized vesicles

J Adamski et al. Biochem J. .

Abstract

Two monoclonal antibodies against the 17 beta-oestradiol dehydrogenase of pig endometrial cells have been used in localization studies with immunogold electron microscopy. The antibodies attach both to a fraction of dehydrogenase-rich cytoplasmic vesicles isolated from homogenates and to vesicles of similar appearance in cells. The vesicles are filled with electron-dense material. Their tagging intensity indicates a high degree of specialization. Endometrial cells from mature animals contain a host of dehydrogenase vesicles, and cells from prepubertal animals only a few. Functional aspects of the novel organelle are discussed.

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