Activation by KRN2391 and nicorandil of glibenclamide-sensitive K+ channels in Xenopus oocytes

Abstract

KRN2391 (N-cyano-N'-(2-nitroxyethyl)-3-pyridine-carboximidamide methanesulfonate) and nicorandil, a new class of K+ channel openers, each with an NO2 moiety, induced outward K+ currents in follicle-enclosed Xenopus oocytes. These K+ currents were suppressed concentration-dependently and reversibly by glibenclamide, phentolamine and trifluoperazine, all known to inhibit ATP-sensitive K+ channels. The nicorandil-induced K+ current was virtually abolished by defolliculation of oocytes, while the KRN2391 response was little affected by defolliculation. These results suggest that Xenopus oocyte has at least two types of glibenclamide-sensitive K+ channels, one is selectively sensitive to KRN2391 and is probably localized in the oocyte, and the other is sensitive to nicorandil and distributed in the follicle cells surrounding an oocyte.