Cloning and sequencing of a cDNA coding for bovine conglutinin

Abstract

A 912 bp bovine cDNA fragment encoding bovine conglutinin was amplified by the RT-PCR technique. cDNA clones encoding the bovine conglutinin were isolated from a bovine liver cDNA library using a specific probe obtained from the PCR product. These cDNAs carry an insert of 1113 bp coding for a protein of 371 amino acid residues with a signal peptide of 20 residues. The deduced amino acid sequence of cDNA agrees with that determined by conventional amino acid sequence analysis. Two polyadenylation signal sequences were detected in the DNA sequence downstream of the 3' end of the gene. Southern blot analysis of total bovine genomic DNA indicated that there is only one copy of the gene encoding bovine conglutinin. Northern blot analysis of bovine tissues showed that conglutinin mRNA of about 1.5 kb is expressed in the liver and also slightly in the lung.