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. 1993 Mar 29;320(1):57-60.
doi: 10.1016/0014-5793(93)81657-l.

Purification and partial characterisation of NADP(+)-linked isocitrate dehydrogenase from rat liver cytosol

H Fatania  1 , K E al-Nassar, V Sidhan
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Free article

Purification and partial characterisation of NADP(+)-linked isocitrate dehydrogenase from rat liver cytosol

H Fatania et al. FEBS Lett. .
Free article

Abstract

NADP(+)-linked isocitrate dehydrogenase from rat liver cytosol was purified (approximately 135-fold) to apparent homogeneity in 27% yield. The purified enzyme has specific activity of 73 units.mg-1. The native enzyme showed an apparent M(r) of 94,000 by gel filtration and was composed of two identical subunits of M(r) 45,000 as judged by SDS/PAGE. In isoelectric focusing, a pI value of 5.7 was estimated for the enzyme.

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