Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Feb 26;612(2):315-9.
doi: 10.1016/0378-4347(93)80179-8.

Determination of boldine in plasma by high-performance liquid chromatography

H Speisky  1 , B K Cassels, S Nieto, A Valenzuela, L J Nuñez-Vergara
Affiliations

Determination of boldine in plasma by high-performance liquid chromatography

H Speisky et al. J Chromatogr. .

Abstract

A sensitive method for the determination of boldine in blood plasma is described. The procedure involves a direct pH-buffered chloroform extraction of boldine from blood plasma, followed by its assay under isocratic conditions by HPLC with UV detection. The extraction recovery is excellent, and sensitivity and precision of the method are very high, when applied to plasma samples containing pharmacologically relevant concentrations of boldine.

PubMed Disclaimer

Publication types