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Comparative Study
. 1993 Apr 1;291 ( Pt 1)(Pt 1):151-5.
doi: 10.1042/bj2910151.

An overview of the kinetic parameters of class B beta-lactamases

A Felici  1 , G Amicosante, A Oratore, R Strom, P Ledent, B Joris, L Fanuel, J M Frère
Affiliations
Comparative Study

An overview of the kinetic parameters of class B beta-lactamases

A Felici et al. Biochem J. .

Abstract

The catalytic properties of three class B beta-lactamases (from Pseudomonas maltophilia, Aeromonas hydrophila and Bacillus cereus) were studied and compared with those of the Bacteroides fragilis enzyme. The A. hydrophila beta-lactamase exhibited a unique specificity profile and could be considered as a rather specific 'carbapenemase'. No relationships were found between sequence similarities and catalytic properties. The problem of the repartition of class B beta-lactamases into sub-classes is discussed. Improved purification methods were devised for the P. maltophilia and A. hydrophila beta-lactamases including, for the latter enzyme, a very efficient affinity chromatography step on a Zn(2+)-chelate column.

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