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. 1993 Apr;39(4):686-9.

Automated spectrophotometric method for determining oxidized and reduced glutathione in liver

J P Teare  1 , N A Punchard, J J Powell, P J Lumb, W D Mitchell, R P Thompson
Affiliations
  • PMID: 8472367

Automated spectrophotometric method for determining oxidized and reduced glutathione in liver

J P Teare et al. Clin Chem. 1993 Apr.

Abstract

An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for GSH, 3.3% for GSSG intraassay CV; 2.15% for GSH, 5% for GSSG interassay CV), and the automation allows large numbers of samples to be conveniently assayed.

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