Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Mar;36(2):107-22.
doi: 10.1016/0165-2427(93)90101-9.

Isolation and functional studies on feline bone marrow derived macrophages

S L Daniel  1 , A M Legendre, R N Moore, B T Rouse
Affiliations

Isolation and functional studies on feline bone marrow derived macrophages

S L Daniel et al. Vet Immunol Immunopathol. 1993 Mar.

Abstract

In this report, we describe an in vitro culture method for feline bone marrow cells, which yields large numbers of quiescent macrophages after 14 days of culture. The bulk of the cultured cell population consists of macrophages as assessed by morphology, macrophage specific cytochemistry, and phagocytosis. The remaining cells were lymphocytes, bone marrow stromal cells, fibroblasts and occasional polymorphonuclear leukocytes. While resting cells produced no detectable interleukin 1, stimulation with lipopolysaccharide (LPS) induced the production of biologically active interleukin 1. After 6 h LPS stimulation, mRNA for tumor necrosis factor alpha and interleukin 1 beta was detectable. The absence of mRNA in unstimulated cells indicates cultured macrophages were not activated until stimulated by LPS or plastic adherence. This approach provides a useful means to measure potential modulatory effects by virus infections or other agents upon feline macrophage gene expression.

PubMed Disclaimer

References

    1. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K., Wang-Iverson P., Bonitz S.G., editors. Short Protocols in Molecular Biology. Greene Publishing Associates and Wiley-Interscience; New York: 1989. pp. 335–358.
    1. Brunner D., Pedersen N.C. Infection of peritoneal macrophages in vitro and in vivo with feline immunodeficiency virus. J. Virol. 1989;63:5483–5488. - PMC - PubMed
    1. Chirgwin T., Przybyla A.E., MacDonald R.J., Rutter W.J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979;18:5294–5299. - PubMed
    1. Daniel S.L., Brenner C.A., Legendre A.M., Solomon A., Rouse B.T. Feline cytokines TNFα and IL-1β: PCR cloning and sequencing of cDNA. Anim. Biotech. 1992 in press.
    1. Dorshkind K. Regulation of hemopoiesis by bone marrow stromal cells and their products. Annu. Rev. Immunol. 1990;8:111–137. - PubMed

Publication types

LinkOut - more resources