Differential organization of synapses immunoreactive to phenylethanolamine-N-methyltransferase or neuropeptide Y in the parvicellular compartments of the hypothalamic paraventricular nucleus of the rat

Abstract

The parvicellular compartments of the paraventricular nucleus of the hypothalamus (pPVN) contains particularly high concentrations of neuropeptide (NPY)-containing fibres of two main cellular origins including (i) neurons of the medulla oblongata, most of which co-store phenylethanolamine-N-methyltransferase (PNMT), the enzyme characterizing adrenergic neurons, and (ii) non-catecholaminergic neurons of the mediobasal hypothalamus. The aim of the present study is to compare the fine organization of the two types of axons terminating in the pPVN. Immunocytochemistry at light and electron microscope levels was used to study both the density and the ultrastructural organization of NPY- and PNMT-immunoreactive fibres in the pPVN of animals bearing surgical lesions disrupting axonal pathways from the hindbrain or from the sublying mediobasal hypothalamus. The brainstem knife-cut induced a strong decrease (65%) in the numerical density of PNMT fibres innervating the pPVN, but was without significant effects on the density of NPY fibres. On the other hand, the hypothalamic knife-cut induced an 80% decrease in the density of NPY fibres within the PVN without affecting the number of PNMT fibres. The electron microscope study showed that in the control pPVN contralateral to the lesions, the majority (64%) of PNMT synapses were asymmetric axo-dendritic synapses, whereas the majority (67%) of NPY synapses form symmetric contacts with both dendrites and perikarya of the hypothalamic nucleus. By contrast, after a hypothalamic knife-cut, the majority (66%) of NPY synapses identified in the pPVN exhibited features of asymmetric synapses. These data indicate that the large majority of NPY-immunoreactive fibres detected within the pPVN arise from non-catecholaminergic neurons located in the mediobasal hypothalamus and mainly form symmetric synapses on neurons of the pPVN, whereas only a minority of them arise from hindbrain regions, and like PNMT fibres innervating this nucleus preferentially form asymmetric axo-dendritic synapses.