Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Mar;59(3):927-32.
doi: 10.1128/aem.59.3.927-932.1993.

Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus

S Paavilainen  1 , J Hellman, T Korpela
Affiliations

Purification, characterization, gene cloning, and sequencing of a new beta-glucosidase from Bacillus circulans subsp. alkalophilus

S Paavilainen et al. Appl Environ Microbiol. 1993 Mar.

Abstract

An intracellular beta-glucosidase was purified from cell extracts of Bacillus circulans subsp. alkalophilus by NAD affinity and high-performance anion-exchange chromatographies. The enzyme was active against a wide range of aryl-beta-glucosides and beta-linked disaccharides. The structural gene for beta-glucosidase was cloned in Escherichia coli. The beta-glucosidase gene consisted of an open reading frame of 1,350 bp encoding a protein of 450 amino acids with a calculated M(r) of 51,303. The enzyme exhibited from 45 to 66% identity with five bacterial beta-glucosidases.

PubMed Disclaimer

References

    1. J Gen Microbiol. 1989 Jul;135(7):1833-46 - PubMed
    1. Proc Natl Acad Sci U S A. 1989 Nov;86(21):8247-51 - PubMed
    1. Gene. 1990 Sep 28;94(1):89-94 - PubMed
    1. Annu Rev Microbiol. 1990;44:219-48 - PubMed
    1. Gene. 1990 Oct 30;95(1):31-8 - PubMed

Publication types

MeSH terms

LinkOut - more resources