Methods of radical prostatectomy specimen processing: a novel technique for harvesting fresh prostate cancer tissue and review of processing techniques
- PMID: 8483892
Methods of radical prostatectomy specimen processing: a novel technique for harvesting fresh prostate cancer tissue and review of processing techniques
Abstract
Standard processing techniques for radical prostatectomy specimens uniformly require initial formalin fixation. Fresh prostate cancer tissue is needed for many types of prostate cancer research and may be needed for diagnostic purposes in the future. We describe a method for processing radical prostatectomy specimens that allows pre-fixation sectioning and harvest of fresh prostate cancer tissue. Because of increased difficulty interpreting capsular margin sections, however, we do not recommend it for routine use. We review other methods of radical prostatectomy specimen processing and provide an algorithm for their appropriate use.
Similar articles
-
Obtaining fresh prostate cancer tissue for research: a novel biopsy needle and sampling technique for radical prostatectomy specimens.Prostate. 2005 Sep 1;64(4):382-6. doi: 10.1002/pros.20264. Prostate. 2005. PMID: 15754345 Clinical Trial.
-
Cryopreservation of prostate cancer tissue during routine processing of fresh unfixed prostatectomy specimen: demonstration and validation of a new technique.Prostate. 2009 Feb 1;69(2):191-7. doi: 10.1002/pros.20868. Prostate. 2009. PMID: 18973174
-
Assessment of radical prostatectomy specimens and diagnostic reporting of pathological findings.Pathologica. 2001 Jun;93(3):226-32. Pathologica. 2001. PMID: 11433618
-
Prognostic factors and reporting of prostate carcinoma in radical prostatectomy and pelvic lymphadenectomy specimens.Scand J Urol Nephrol Suppl. 2005 May;(216):34-63. doi: 10.1080/03008880510030932. Scand J Urol Nephrol Suppl. 2005. PMID: 16019758 Review.
-
Handling of radical prostatectomy specimens.Histopathology. 2012 Jan;60(1):118-24. doi: 10.1111/j.1365-2559.2011.04002.x. Histopathology. 2012. PMID: 22212081 Review.
Cited by
-
Natural history of pathologically organ-confined (pT2), Gleason score 6 or less, prostate cancer after radical prostatectomy.Urology. 2008 Jul;72(1):172-6. doi: 10.1016/j.urology.2007.10.055. Epub 2008 Mar 4. Urology. 2008. PMID: 18304619 Free PMC article.
-
Trefoil factor 3 overexpression in prostatic carcinoma: prognostic importance using tissue microarrays.Prostate. 2004 Nov 1;61(3):215-27. doi: 10.1002/pros.20095. Prostate. 2004. PMID: 15368473 Free PMC article.
-
Cancer/Testis Antigens as potential predictors of biochemical recurrence of prostate cancer following radical prostatectomy.J Transl Med. 2011 Sep 14;9:153. doi: 10.1186/1479-5876-9-153. J Transl Med. 2011. PMID: 21917134 Free PMC article.
-
Integrity of prostatic tissue for molecular analysis after robotic-assisted laparoscopic and open prostatectomy.Urology. 2007 Aug;70(2):328-32. doi: 10.1016/j.urology.2007.04.005. Urology. 2007. PMID: 17826499 Free PMC article.
-
Cancer/testis antigen PAGE4, a regulator of c-Jun transactivation, is phosphorylated by homeodomain-interacting protein kinase 1, a component of the stress-response pathway.Biochemistry. 2014 Mar 18;53(10):1670-9. doi: 10.1021/bi500013w. Epub 2014 Mar 5. Biochemistry. 2014. PMID: 24559171 Free PMC article.
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Medical