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Comparative Study
. 1993 Apr 15;291 ( Pt 2)(Pt 2):595-600.
doi: 10.1042/bj2910595.

The sub-cellular localization of annexin V in cultured chick-embryo fibroblasts

J J Koster  1 , C M Boustead, C A Middleton, J H Walker
Affiliations
Comparative Study

The sub-cellular localization of annexin V in cultured chick-embryo fibroblasts

J J Koster et al. Biochem J. .

Abstract

The Ca(2+)- and phospholipid-binding protein, annexin V, has been shown by an immune assay to represent 0.4% of total cell protein in cultured chick-embryo fibroblasts. Immunofluorescent localization studies indicate that in primary cultures the protein is abundant in the cytoplasm of the cells and also extends into the nucleus. Nuclear staining is no longer detectable, however, in approx. 25% of the cells following sub-culture. Sub-populations of annexin V are associated with cytoskeletal structures and with the inner face of the plasma membrane in a Ca(2+)-independent manner. In addition, we report results indicating the secretion of annexin V from this cell type.

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References

    1. J Neurochem. 1982 Sep;39(3):815-23 - PubMed
    1. Biochem J. 1993 Apr 15;291 ( Pt 2):601-8 - PubMed
    1. J Cell Biol. 1983 Nov;97(5 Pt 1):1601-11 - PubMed
    1. EMBO J. 1983;2(10):1701-5 - PubMed
    1. EMBO J. 1984 Jan;3(1):227-33 - PubMed

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