[Three mutant forms of ribosomal protein L7/L12]

Abstract

Three mutant forms of the ribosomal protein L7/L12 with Ser1, Met14, and Met26 replacements with Tyr were constructed for studying the N-terminal domain of the protein. Three point mutations in the gene L7/L12 on the phage M13mp18 were generated. Recombinant plasmids containing the mutant genes were constructed on the base of expression vector pKK223-3 and plasmid pUC19. The mutant proteins were expressed in Escherichia coli cells, and methods of their purification were developed. It was found that the mutant proteins L7/L12 do not differ from the wild-type protein L7/L12 and represent a suitable object for 1H-NMR study of the L7/L12 protein. The three mutant proteins bind with the E. coli ribosome.