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Comparative Study
. 1993 May;175(10):3220-3.
doi: 10.1128/jb.175.10.3220-3223.1993.

Specific in vitro guanylylation of a 43-kilodalton membrane-associated protein of Streptomyces coelicolor

A J Obaya  1 , J Guijarro
Affiliations
Comparative Study

Specific in vitro guanylylation of a 43-kilodalton membrane-associated protein of Streptomyces coelicolor

A J Obaya et al. J Bacteriol. 1993 May.

Abstract

Incubation of [alpha-32P]GTP with cellular extracts or membranes of Streptomyces coelicolor labels a protein of 43 kDa, which was also labeled with [8,5'-3H]GTP but not with [alpha-32P]ATP or [gamma-32P]GTP. Radioactivity remained associated with this protein after boiling in 0.1 N NaOH, but it was dissociated after incubation in 0.1 N HCl or hydroxylamine. Chromatographic analysis of the HCl-dissociated compound showed that GMP was the covalently bound nucleotide. Furthermore, guanylylation appeared to be reversible and to take place by a pyrophosphorylytic mechanism. Guanylylation was more efficient at low temperatures. Several Streptomyces species showed a guanylylated protein with a similar molecular mass.

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