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. 1977 Feb 1;161(2):419-24.
doi: 10.1042/bj1610419.

The isolation of minimally degraded hyaluronate from rat skin

J M Mathieson, R H Pearce

The isolation of minimally degraded hyaluronate from rat skin

J M Mathieson et al. Biochem J. .

Abstract

Hyaluronate was isolated quantitatively from fresh rat skin by homogenization in an Edebo [J. Biochem. Microbiol. Technol. Eng. 2, (1960) 453-479] press, extraction with buffered saline, selective elution from DEAE-cellulose, and gel chromatography on Sephadex G-200. Traces of a protein contaminant remained.

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