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. 1977 Jan 15;162(1):201-3.
doi: 10.1042/bj1620201.

The turnover of hamster fibroblast lysosomal beta-D-glucuronidase

M J Warburton, C H Wynn

The turnover of hamster fibroblast lysosomal beta-D-glucuronidase

M J Warburton et al. Biochem J. .

Abstract

The half-life of hamster fibroblast beta-D-glucuronidase (EC 3.2.1.31) was estimated to be 4-5 days by measuring the decay with time of the radioactivity in beta-D-glucuronidase isolated from cells grown in the presence of 14C-labelled amino acids. A new affinity-chromatographic procedure for the purification of beta-D-glucuronidase is described.

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