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. 1993 May;31(5):1379-81.
doi: 10.1128/jcm.31.5.1379-1381.1993.

Development of an enzyme-linked immunosorbent assay method for typing and quantitation of Klebsiella pneumoniae lipopolysaccharide: application to serotype O1

S Albertí  1 , S Hernández-Allés, J Gil, J Reina, J Martínez-Beltrán, S Camprubí, J M Tomás, V J Benedí
Affiliations

Development of an enzyme-linked immunosorbent assay method for typing and quantitation of Klebsiella pneumoniae lipopolysaccharide: application to serotype O1

S Albertí et al. J Clin Microbiol. 1993 May.

Abstract

We describe a method for the typing and quantitation of Klebsiella pneumoniae serotype O1 lipopolysaccharide (LPS) based on inhibition in an enzyme-linked immunosorbent assay of a reaction of known O1 LPS antigen and anti-O1 antibody by unknown LPS extracts. Serotype O1 was found in 32% of the 124 K. pneumoniae clinical isolates tested, showing that this serotype is frequent among the eight O serotypes which have been described previously.

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