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. 1993 Jan;106(1):31-4.

Cloning of a DNA probe and its application in the detection of Plasmodium falciparum. A preliminary report

K Y Liu  1 , B C Huang, H H Zhang, Y B Liu, R Q Kong, Y L Cheng
Affiliations
  • PMID: 8504679

Cloning of a DNA probe and its application in the detection of Plasmodium falciparum. A preliminary report

K Y Liu et al. Chin Med J (Engl). 1993 Jan.

Abstract

The clones containing parasite DNA fragments were screened from a genomic DNA library of Plasmodium falciparum FCC1/HN isolate. A DNA probe derived from clone pBF4 consisting of 3 kilobase pairs hybridizes specifically with P. falciparum DNA but not with human DNA, P. cynomolgi DNA or P. berghei DNA. The nick translated radiolabelled probe can detect 10 pg purified P. falciparum DNA and a 0.001% parasitemia after 24 hours of film exposure. The probe reacts with all microscopically diagnosed P. falciparum samples and 3 of 41 P. vivax samples as well but not with any of 10 human DNA samples.

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