Colocalization of peptide hormones in neuroendocrine cells of human fetal and newborn lungs: an electron microscopic study

Abstract

This study investigated the colocalization of the peptide hormones bombesin or calcitonin with calcitonin gene related peptide (CGRP) in neuroendocrine cells (NE) in the lungs of human fetuses of varying gestational ages and in the lungs of newborn infants who died with acute or chronic lung disease in the first weeks or months after birth. Double immunolabeling of dense core granules for these peptides was also studied in this same patient population. On-grid double gold immunolabeling was carried out on 29 subjects using anti-bombesin and anti-CGRP and on 22 subjects using anti-calcitonin and anti-CGRP as primary antibodies, the secondary antibodies being labeled with different-size gold spheres. Colocalization of both bombesin and calcitonin with CGRP was demonstrated, not only in the same NE cell, but also on the same dense core granule. Colocalization was rarely found in normal fetuses, and most frequently found in newborn infants with acute lung disease, usually hyaline membrane disease (HMD), or with the development of chronic lung disease in the first weeks or months after birth. Double labeling of the same dense core granules might imply action of peptides in concert, or perhaps one peptide acting in a paracrine role (e.g., on bronchial or bronchiolar smooth muscle) and the second peptide acting in an autocrine fashion on the parent cell (e.g., in the regulation of granule production or release).