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. 1995 Nov 27;376(1-2):81-6.
doi: 10.1016/0014-5793(95)01253-1.

Characterisation of an Arabidopsis thaliana cDNA encoding glutathione synthetase

M R Rawlins  1 , C J Leaver, M J May
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Free article

Characterisation of an Arabidopsis thaliana cDNA encoding glutathione synthetase

M R Rawlins et al. FEBS Lett. .
Free article

Abstract

An Arabidopsis thaliana cDNA (AtGSHS) encoding a protein with high primary sequence identity to cDNAs previously isolated from Xenopus laevis (42%), Schizosaccharomyces pombe (40%), Rattus norvegicus (40%) and Homo sapiens (37%) encoding glutathione synthetase (EC 6.3.2.3) has been isolated by functional complementation of an Escherichia coli mutant deficient in this enzyme. AtGSHS is encoded by a single gene, GSHB, as determined by Southern blot analysis and the corresponding mRNA is abundant in both roots and leaves of Arabidopsis.

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