Spontaneous deletion mutants of the Lactococcus lactis temperate bacteriophage BK5-T and localization of the BK5-T attP site
- PMID: 8526525
- PMCID: PMC167718
- DOI: 10.1128/aem.61.11.4105-4109.1995
Spontaneous deletion mutants of the Lactococcus lactis temperate bacteriophage BK5-T and localization of the BK5-T attP site
Abstract
Spontaneous deletion mutants of the temperate lactococcal bacteriophage BK5-T were obtained when the phage was grown vegetatively on the indicator strain Lactococcus lactis subsp. cremoris H2. One deletion mutant was unable to form stable lysogens, and analysis of this mutant led to the identification of the BK5-T attP site and the integrase gene (int). The core sequences of the BK5-T attP and host attB regions are conserved in a number of lactococcal phages and L. lactis strains.
Similar articles
-
Use of the integration elements encoded by the temperate lactococcal bacteriophage TP901-1 to obtain chromosomal single-copy transcriptional fusions in Lactococcus lactis.Appl Environ Microbiol. 1999 Feb;65(2):752-8. doi: 10.1128/AEM.65.2.752-758.1999. Appl Environ Microbiol. 1999. PMID: 9925612 Free PMC article.
-
Identification of prophage genes expressed in lysogens of the Lactococcus lactis bacteriophage BK5-T.Appl Environ Microbiol. 1995 Nov;61(11):4099-104. doi: 10.1128/aem.61.11.4099-4104.1995. Appl Environ Microbiol. 1995. PMID: 8526524 Free PMC article.
-
Identification of int and attP on the genome of lactococcal bacteriophage Tuc2009 and their use for site-specific plasmid integration in the chromosome of Tuc2009-resistant Lactococcus lactis MG1363.Appl Environ Microbiol. 1994 Jul;60(7):2324-9. doi: 10.1128/aem.60.7.2324-2329.1994. Appl Environ Microbiol. 1994. PMID: 8074513 Free PMC article.
-
Sequence analysis of the Lactococcus lactis temperate bacteriophage BK5-T and demonstration that the phage DNA has cohesive ends.Appl Environ Microbiol. 1995 Nov;61(11):4089-98. doi: 10.1128/aem.61.11.4089-4098.1995. Appl Environ Microbiol. 1995. PMID: 8526523 Free PMC article.
-
Streptomyces temperate bacteriophage integration systems for stable genetic engineering of actinomycetes (and other organisms).J Ind Microbiol Biotechnol. 2012 May;39(5):661-72. doi: 10.1007/s10295-011-1069-6. Epub 2011 Dec 13. J Ind Microbiol Biotechnol. 2012. PMID: 22160317 Review.
Cited by
-
Use of the integration elements encoded by the temperate lactococcal bacteriophage TP901-1 to obtain chromosomal single-copy transcriptional fusions in Lactococcus lactis.Appl Environ Microbiol. 1999 Feb;65(2):752-8. doi: 10.1128/AEM.65.2.752-758.1999. Appl Environ Microbiol. 1999. PMID: 9925612 Free PMC article.
-
Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor.J Bacteriol. 2002 Dec;184(23):6532-44. doi: 10.1128/JB.184.23.6532-6543.2002. J Bacteriol. 2002. PMID: 12426341 Free PMC article.
-
Defective site-specific integration elements are present in the genome of virulent bacteriophage LL-H of Lactobacillus delbrueckii.Appl Environ Microbiol. 1996 May;62(5):1847-51. doi: 10.1128/aem.62.5.1847-1851.1996. Appl Environ Microbiol. 1996. PMID: 8633887 Free PMC article.
-
Sequence analysis and molecular characterization of the Lactococcus lactis temperate bacteriophage BK5-T.Appl Environ Microbiol. 2001 Aug;67(8):3564-76. doi: 10.1128/AEM.67.8.3564-3576.2001. Appl Environ Microbiol. 2001. PMID: 11472933 Free PMC article.
-
A resolvase-like protein is required for the site-specific integration of the temperate lactococcal bacteriophage TP901-1.J Bacteriol. 1996 Sep;178(17):5164-73. doi: 10.1128/jb.178.17.5164-5173.1996. J Bacteriol. 1996. PMID: 8752334 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources