Detection of secondary amines on solid phase

Abstract

The detection of secondary amino groups in building blocks attached to solid phase using tetrachlorobenzoquinone (chloranil) was re-examined. A new procedure, about ten times more sensitive than the original method, has been developed. Except for N(p-tolyl)glycine, all secondary amino groups that were examined were detected reliably in a substitution range down to 2.8 micro eq/g. Protected imidazole in His(Trt) did not interfere with the detection method.