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. 1979 Jan;76(1):106-10.
doi: 10.1073/pnas.76.1.106.

Expression in Escherichia coli of chemically synthesized genes for human insulin

D V Goeddel, D G Kleid, F Bolivar, H L Heyneker, D G Yansura, R Crea, T Hirose, A Kraszewski, K Itakura, A D Riggs

Expression in Escherichia coli of chemically synthesized genes for human insulin

D V Goeddel et al. Proc Natl Acad Sci U S A. 1979 Jan.

Abstract

Synthetic genes for human insulin A and B chains were cloned separately in plasmid pBR322. The cloned synthetic genes were then fused to an Escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. The insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. Complete purification of the A chain and partial purification of the B chain were achieved. These products were mixed, reduced, and reoxidized. The presence of insulin was detected by radioimmunoassay.

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