Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Dec;61(12):4167-71.
doi: 10.1128/aem.61.12.4167-4171.1995.

Polymer production by Klebsiella pneumoniae 4-hydroxyphenylacetic acid hydroxylase genes cloned in Escherichia coli

A Gibello  1 , E Ferrer, J Sanz, M Martin
Affiliations

Polymer production by Klebsiella pneumoniae 4-hydroxyphenylacetic acid hydroxylase genes cloned in Escherichia coli

A Gibello et al. Appl Environ Microbiol. 1995 Dec.

Abstract

The expression of Klebsiella pneumoniae hpaA and hpaH genes, which code for 4-hydroxyphenylacetic acid hydroxylase in Escherichia coli K-12 derivative strains, is associated with the production of a dark brown pigment in the cultures. This pigment has been identified as a polymer which shows several of the characteristics reported for microbial melanins and results from the oxidative activity of 4-hydroxyphenylacetic acid hydroxylase on some dihydroxylated compounds to form o-quinones. A dibenzoquinone is formed from the oxidation of different mono- or dihydroxylated aromatic compounds by the enzyme prior to polymerization. We report a hydroxylase activity, other than tyrosinase, that is associated with the synthesis of a bacterial melanin.

PubMed Disclaimer

References

    1. Acta Vet Scand. 1972;13(2):251-9 - PubMed
    1. Bacteriol Rev. 1972 Dec;36(4):525-57 - PubMed
    1. J Bacteriol. 1975 Jan;121(1):272-85 - PubMed
    1. J Med Microbiol. 1975 Feb;8(1):199-203 - PubMed
    1. J Bacteriol. 1975 Jun;122(3):866-73 - PubMed

Publication types

MeSH terms