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. 1977 Apr;22(1):97-101.
doi: 10.1128/JVI.22.1.97-101.1977.

Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system

C W Clinkscales, M A Bratt, T G Morrison

Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system

C W Clinkscales et al. J Virol. 1977 Apr.

Abstract

We have isolated 18S RNA from cytoplasmic extracts of Newcastle disease virus-infected Chinese hamster ovary cells and tested its ability to direct protein synthesis in extracts derived from wheat germ. The products of the cell-free reaction directed by this RNA contain polypeptides that comigrate with NP, M,F, and 47K roteins from virions. In addition, the products contain a polypeptide (67K) that migrates on polyacrylamide gels slightly faster than the HN protein from virions. Tryptic peptide analysis of the cell-free products and proteins from virions confirms their identity.

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References

    1. J Gen Virol. 1976 Oct;33(1):117-23 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 - PubMed
    1. Biochemistry. 1974 Aug 13;13(17):3606-15 - PubMed
    1. J Virol. 1970 Nov;6(5):677-84 - PubMed
    1. Nat New Biol. 1971 Jun 23;231(25):229-32 - PubMed

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